|
| Status |
Public on Oct 01, 2005 |
| Title |
ice1_3H Cold_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
A mutant defective in an upstream transcription factor required for chilling and freezing tolerance as described in Chinnusamy, V., Ohta, M., Kanrar, S., Lee, B.H., Hong, X.H., Agarwal, M., and Zhu, J.K. (2003). ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev. 17, 1043-1054.
|
| Treatment protocol |
Treated at 0C for 3 hours just prior to harvesting.
|
| Growth protocol |
The wild-type and ice1 mutant seeds were plated on MS agar plates supplemented with 3% sucrose. Seedlings were grown at 22C with 16-h light and 8-h dark cycles for 2 weeks before being treated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit, Qiagen
|
| Label |
Biotin
|
| Label protocol |
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
|
| |
|
| Hybridization protocol |
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
|
| Scan protocol |
Followed users manual: http://www.affymetrix.com/support/downloads/manuals/expression_s21_manual.pdf
|
| Description |
Target value (TGT value) was set to 500 global scaling.
|
| Data processing |
Microarray data were extracted from scanned GeneChip images and analyzed by use of Microarray Suite version 5.0.1
|
| |
|
| Submission date |
Sep 18, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jian-Kang Zhu |
| E-mail(s) |
[email protected]
|
| Phone |
951-827-7117
|
| Fax |
951-827-7115
|
| URL |
http://www.faculty.ucr.edu/~jkzhu
|
| Organization name |
University of California
|
| Department |
Botany and Plant Sciences
|
| Street address |
2150 Batchelor Hall
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE3326 |
ICE1 regulation of the Arabidopsis Cold-Responsive Transcriptome |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
