Biotinylated cRNA was prepared by using the Ambion Illumina RNA Amplification Kit (Ambion, Austin, TX, USA). 日野案）Total RNA was isolated by using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Valencia, CA, USA), and genomic DNA in the RNA samples was digested by RNase-free DNase I.
Label
streptavidin-Cy3
Label protocol
The BeadChips were labeled with streptavidin–Cy3 (GE Healthcare, Chicago, IL, USA). 日野案）Biotinylated cRNA was prepared by using the Ambion Illumina RNA Amplification Kit (Ambion, Austin, TX, USA).
Hybridization protocol
The biotinylated cRNA were hybridized to Human 6v2 Expression BeadChips (Illumina, San Diego, CA, USA). 日野案）The biotinylated cRNA were hybridized to Human 6v2 Expression BeadChips (Illumina, San Diego, CA, USA). The BeadChips were then labeled with streptavidin–Cy3 (GE Healthcare, Chicago, IL, USA).
Scan protocol
Standard Illumina scanning protocol
Description
replication
Data processing
Signal intensity (SI) of each bead was measured and normalized using average normalization by BeadStudio software Ver. 3.1 (Illumina).
