|
| Status |
Public on Dec 21, 2012 |
| Title |
5_d21 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral white blood cells, D21
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
disease state: non-metastatic prostate cancer
treatment: external beam radiation therapy (EBRT)
tissue: peripheral blood
cell type: white blood cells
time of wbc and fatigue score collection: D21
individual: 5
|
| Treatment protocol |
No treatment for the cells for this natural history study.
|
| Growth protocol |
Peripheral blood was collected using PAXgene tubes, and then stored immediately to -80 degree freezer until ready for RNA extraction. Cells were not grown or cultured.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen) and purified using an RNeasy mini kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
A total of 5 µg of purified RNA was reverse-transcribed into cDNA using T7-oligo (dT) primer (Affymetrix, Santa Clara, CA) and SuperScript II RT (Invitrogen). The biotin-labeled cRNA was synthesized from cDNA using the BioArray RNA transcript labeling kit (Enzo, Farmingdale, NY) and was followed by fragmentation.
|
| |
|
| Hybridization protocol |
A total of 15 µg of fragmented cRNA was hybridized on an Affymetrix GeneChip (human U133A) at 45ºC for 16 hours at 60 rpm followed by washing/staining procedures.
|
| Scan protocol |
All probe arrays were scanned using the GeneChip scanner 3000, and operating software (MAS 5.0) was used to scan the images and for data acquisition.
|
| Description |
JJB100504_5E
|
| Data processing |
Affymetrix Microarray Suite version 5.0.
|
| |
|
| Submission date |
Jul 01, 2011 |
| Last update date |
Dec 22, 2012 |
| Contact name |
Jennifer Barb |
| E-mail(s) |
[email protected]
|
| Phone |
3014359232
|
| Organization name |
NIH
|
| Department |
DCB
|
| Lab |
MSCL
|
| Street address |
12 South Drive Bldg 12A Room 2001
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE30174 |
Molecular-genetic correlates of fatigue in cancer patients receiving localized external beam radiation therapy |
|
