|
| Status |
Public on Dec 12, 2005 |
| Title |
IFN_4 hours_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
CD8+ DCs purified from mouse spleen
|
| Organism |
Mus musculus |
| Characteristics |
Strain: DBA/2J
Gender: female
Age: 60 days
Tissue: spleen
|
| Treatment protocol |
Mice were treated with 10 µg of Flt3-L for 9 days
|
| Growth protocol |
CD8+ DCs were incubated on medium alone (CTR) or with 200 U/ml of mouse IFN-gamma (IFN) for 4 or 16 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from incubated cells using TRIzol LS Reagent (Invitrogen Life Technologies, Carlsbad, CA). RNA samples were processed according to the Affymetrix Expression Analysis Technical Manual.
|
| Label |
Biotin
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets according to Affymetrix Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
|
| Scan protocol |
Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
|
| Description |
Global transcriptional analysis to explore the gene expression pattern of CD8+ DCs purified from the mouse spleen and treated with IFN-gamma
|
| Data processing |
Robust Multi-Array Average (RMA) procedure
|
| |
|
| Submission date |
Sep 21, 2005 |
| Last update date |
Dec 12, 2005 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
[email protected]
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE3337 |
Gene expression profiling of IDO-competent dendritic cells |
|
