solitary root (slr-1) mutant, root segments of 3 day old seedlings, NPA grown plus 6h NAA treatment
Extracted molecule
total RNA
Extraction protocol
Root segments as described in Himanen et al (2002; Plant Cell, 14, 2339-2351) were dissected from 3 day old slr-1 seedlings, germinated on 10 µM NPA and an additional 6h 10µM NAA treatment. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 5.8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
lateral root inducible segment (between root meristem and hypocotyl)
