|
| Status |
Public on Jul 08, 2011 |
| Title |
SN250PK/SN250BF biological replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Candida albicans planktonic a/alpha cells
|
| Organism |
Candida albicans |
| Characteristics |
strain type: planktonic
|
| Growth protocol |
planktonic, Spider medium, 37 degrees
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Extract protocol is using the Ambion Yeast RNeasy Kit following the manufacturer's protocol [see Nobile et al. 2009 (PMCID: PMC2688839)].
|
| Label |
Cy3
|
| Label protocol |
see Lohse and Johnson, 2010 (PMCID: PMC3057202)
|
| |
|
| Channel 2 |
| Source name |
Candida albicans biofilm a/alpha cells
|
| Organism |
Candida albicans |
| Characteristics |
strain type: biofilm
|
| Growth protocol |
biofilm, 48 hours, Spider medium, 37 degrees
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Extract protocol is using the Ambion Yeast RNeasy Kit following the manufacturer's protocol [see Nobile et al. 2009 (PMCID: PMC2688839)].
|
| Label |
Cy5
|
| Label protocol |
see Lohse and Johnson, 2010 (PMCID: PMC3057202)
|
| |
|
| |
| Hybridization protocol |
see Lohse and Johnson, 2010 (PMCID: PMC3057202)
|
| Scan protocol |
Slides were scanned on a Genepix 4000B Axon Instrument Scanner, and processed using GenePix Pro version 5.1 [see Lohse and Johnson, 2010 (PMCID: PMC3057202)].
|
| Description |
252016610025_530_430
|
| Data processing |
Global Lowess normalization analysis was performed for each array using a Goulphar script (Lemoine et al., 2006) for R (The R Foundation for Statistical Computing) [see Lohse and Johnson, 2010 (PMCID: PMC3057202)].
|
| |
|
| Submission date |
Jul 07, 2011 |
| Last update date |
Jul 08, 2011 |
| Contact name |
Clarissa Jane Nobile |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California - Merced
|
| Department |
Molecular and Cell Biology
|
| Lab |
Clarissa Nobile
|
| Street address |
5200 North Lake Rd
|
| City |
Merced |
| State/province |
CA |
| ZIP/Postal code |
95343 |
| Country |
USA |
| |
|
| Platform ID |
GPL13830 |
| Series (1) |
| GSE30474 |
Expression changes during Candida albicans biofilm formation |
|
