|
| Status |
Public on Mar 31, 2012 |
| Title |
FicP adult head, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila adult head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: FicP
tissue: decapitulated head
genotype: Canton S parents
age: Adult
|
| Treatment protocol |
Flies were anaesthetized briefly by using 5% CO2, then immediately dissected. The tissues dissected were the complete head, severed at the neck from the adult flies and the CNS (developing brain), including the optic lobes from the larvae
|
| Growth protocol |
Flies were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. Flies were collected irrespective of their age and sex, whereas the larvae used were third instar, wandering larvae.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 30007G.
|
| Description |
Drosophila_FicP H3.CEL
|
| Data processing |
RMA (R with Affy package for data normalization).
|
| |
|
| Submission date |
Jul 13, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Hossain Nawaz |
| E-mail(s) |
[email protected]
|
| Phone |
+46858583806
|
| Fax |
+46858583650
|
| Organization name |
Karolinska Institutet
|
| Department |
LABMED
|
| Lab |
KFC/MCG Lab 514
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| State/province |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE30627 |
Expression data from Drosophila Btk29A mutant and revertant neuronal tissue |
|
| Relations |
| Reanalyzed by |
GSE119084 |
