|
| Status |
Public on Aug 15, 2011 |
| Title |
MB231_siRelA_TNF_4h |
| Sample type |
RNA |
| |
|
| Source name |
Breast cancer cell line (MDA-MB-231)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
treatment group: siRelA_TNF_4h
|
| Treatment protocol |
MDA-MB-231 was transfected with siRNA of negative control, EZH2, RelA, and RelB respectively followed by treatment of 10ng/ml TNFalpha for 4 hours.
|
| Growth protocol |
MDA-MB-231 was cultured in were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and 5000U/mL penicillin/streptomycin (Invitrogen) and maintained at 37 °C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen) and purified with the RNeasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
standard Illumina protocol
|
| Scan protocol |
standard Illumina protocol
|
| Description |
siRelA TNF4h
|
| Data processing |
Illumina Inc. BeadStudio version 3.2.7; Shift to 75 percentile
|
| |
|
| Submission date |
Jul 14, 2011 |
| Last update date |
Aug 15, 2011 |
| Contact name |
Peiyong Guan |
| E-mail(s) |
[email protected]
|
| Organization name |
Genome Institute of Singapore
|
| Street address |
60 Biopolis Street, Genome, #02-01
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE30670 |
Context-Specific Regulation of NF-κB Target Gene Expression by EZH2 in Breast Cancers |
|
