For preparation of islet cells : according to Ouziel-Yahalom L., et al., Biochem Biophys Res Commun (2006) 341:291-298
Extracted molecule
total RNA
Extraction protocol
according to the manufacturer's protocol (Trizol, Sigma-Aldrich)
Label
biotin
Label protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Hybridization protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Description
gene expression data of intact islet cells
Data processing
Gene-level analyses was performed. RMA normalization, summarization by mean and batch effect removal, all were done by Partek Genomics suite (version 6.5) Copyright.
