Redifferentiation Cocktail (RC) treatment consisted of 6 days in SFM supplemented with 25 mM D-glucose, N2 and B27 supplements (Stem Cell Technologies), 10 mM nicotinamide (Sigma), 8 nM exendin-4 (Acris), and 8 nM activin A (Peprotech), followed by 2 days in the same medium lacking activin A and containing a reduced glucose concentration (5.6 mM).
Extracted molecule
total RNA
Extraction protocol
according to the manufacturer's protocol (Trizol, Sigma-Aldrich)
Label
biotin
Label protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Hybridization protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Description
gene expression data of re-differentiation cells
Data processing
Gene-level analyses was performed. RMA normalization, summarization by mean and batch effect removal, all were done by Partek Genomics suite (version 6.5) Copyright.
