|
| Status |
Public on Mar 17, 2024 |
| Title |
RNA_WT_MII_Rep9 |
| Sample type |
SRA |
| |
|
| Source name |
MII oocyte
|
| Organism |
Mus musculus |
| Characteristics |
cell type: MII oocyte
genotype: Wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
FGOs were harvested from adult ovaries (10 to 12 weeks old) by needle puncture and cumulus cells were removed by capillary washing.
RNA-seq library was generated from a single FGO or MII oocyte using SMART-SeqHT Kit (Takara) according to the manufacturer's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
SMART-seq (single cell)
|
| Data processing |
RNA-seq reads were aligned against the mouse mm10 genome assembly using STAR (Dobin et al. 2013). The mapped reads were counted using HTSeq (Anders et al. 2015)
Assembly: mm10
Supplementary files format and content: text file format
|
| |
|
| Submission date |
Jul 25, 2023 |
| Last update date |
Mar 17, 2024 |
| Contact name |
Naoki Kubo |
| E-mail(s) |
[email protected]
|
| Organization name |
Kyushu University
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE238226 |
Combinational effect of the ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells [RNA-seq] |
| GSE238228 |
Combinational effect of the ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells |
|
| Relations |
| BioSample |
SAMN36705828 |
| SRA |
SRX21156823 |
