|
Status |
Public on Jul 27, 2023 |
Title |
SI_IEL_TRM_HC_R2 |
Sample type |
SRA |
|
|
Source name |
Small Intestine CD8 T cells
|
Organism |
Mus musculus |
Characteristics |
tissue: Small Intestine CD8 T cells cell type: P14 CD8 T cells genotype: C57/BL6 treatment: High cholesterol-containing diet for 7 days
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Ultra-low-input RNA Seq was performed on sort purified cells, polyadenylated mRNA was converted to cDNA. Illumina adapters were added and product was PCR amplified for sequencing Immgen ULI-RNAseq
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Aligned with STAR 2.5.4a to mouse genome Gene-level quantification calculated by featureCounts Assembly: mm10 Supplementary files format and content: Gene counts by sample as an .xlsx file
|
|
|
Submission date |
Jul 25, 2023 |
Last update date |
Jul 27, 2023 |
Contact name |
Miguel Reina-Campos |
E-mail(s) |
[email protected]
|
Organization name |
La Jolla Institute for Immunology
|
Lab |
Reina
|
Street address |
9420 Athena Circle
|
City |
La Jolla |
State/province |
California |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE207044 |
Metabolic Programs of T Cell Tissue Residency Empower Tumor Immunity |
GSE238249 |
Metabolic Programs of T Cell Tissue Residency Empower Tumor Immunity [RNA-seq II] |
|
Relations |
BioSample |
SAMN36707035 |
SRA |
SRX21157812 |