|
| Status |
Public on Oct 29, 2011 |
| Title |
GST_RNAi_replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
S2 cell line, control dsRNA
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2
dsrna treatment: GST dRNA
|
| Treatment protocol |
1.5x10exp6 cells were treated with 10 µg dsRNA targeted against NSL1 or glutathione-S-transferase (control) for 1 hour in serum-free medium. Then, serum-containing Schneider medium was added, and cells were incubated for 6 days at 26°C before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen), followed by a purification using the RNeasy kit (Quiagen). RNA quality was checked using the Bioanalyzer (Agilent).
|
| Label |
biotin
|
| Label protocol |
Performed by Dietmar Martin and colleagues (Laboratory of Patrick Cramer, Gene Center, Munich, Germany).
|
| |
|
| Hybridization protocol |
Performed by Dietmar Martin and colleagues (Laboratory of Patrick Cramer, Gene Center, Munich, Germany).
|
| Scan protocol |
Performed by Dietmar Martin and colleagues (Laboratory of Patrick Cramer, Gene Center, Munich, Germany).
|
| Description |
Gene expression data from control RNAi-treated S2 cells.
|
| Data processing |
Raw signals were processed using the R package “gcrma” (Wu and Irizarry, 2005).
|
| |
|
| Submission date |
Jul 27, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Christian Feller |
| E-mail(s) |
[email protected]
|
| Phone |
089-2180-75-436
|
| URL |
http://www.molekularbiologie.abi.med.uni-muenchen.de/index.html
|
| Organization name |
Adolf-Butenandt Institute at the LMU University Munich
|
| Department |
Molecular Biology
|
| Lab |
Peter B. Becker
|
| Street address |
Schillerstraße 44
|
| City |
Munich |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE30990 |
The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset (RNAi) |
| GSE30991 |
The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset |
|
| Relations |
| Reanalyzed by |
GSE119084 |
