GEO help: Mouse over screen elements for information.
Status
Public on Jul 14, 2006
Title
CSB null replicate 1 U133A
Sample type
RNA
Source name
human Cockayne syndrome skin fibroblasts
Organism
Homo sapiens Characteristics
Patient: CS1AN
Extracted molecule
polyA RNA
Extraction protocol
Stable lines of hTERT-immortalized, mock-rescued fibroblasts were grown to 60-80% confluence, and total RNA collected in RNeasy spin columns (Qiagen). Samples were enriched for PolyA RNA using Oligotex (Qiagen).
Label
biotin
Label protocol
Approximately 2 mcirog of polyA RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Cockayne syndrome (CSB) fibroblasts mock-rescued with expression of eGFP cDNA
Data processing
Affymetrix Microarray Suite version 5.0, values scaled to Target Signal of 800
Submission date
Oct 03, 2005
Last update date
Jul 14, 2006
Contact name
John C Newman
E-mail(s)
[email protected] Organization name
University of Washington
Department
Biochemistry
Lab
Alan Weiner
Street address
1959 NE Pacific St.
City
Seattle
State/province
WA
ZIP/Postal code
98195
Country
USA
Platform ID
GPL96 Series (1)
GSE3407 Cockayne syndrome (CSB) fibroblasts
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
516
P
0.004484
AFFX-BioB-M_at
859.8
P
0.000127
AFFX-BioB-3_at
452.3
P
0.000581
AFFX-BioC-5_at
1424.7
P
0.000195
AFFX-BioC-3_at
819.6
P
0.00039
AFFX-BioDn-5_at
1189.5
P
0.00011
AFFX-BioDn-3_at
8006.6
P
0.000195
AFFX-CreX-5_at
12488.9
P
4.4e-05
AFFX-CreX-3_at
18313
P
4.4e-05
AFFX-DapX-5_at
10.2
A
0.559354
AFFX-DapX-M_at
118
A
0.156732
AFFX-DapX-3_at
27.4
A
0.794268
AFFX-LysX-5_at
4.1
A
0.860518
AFFX-LysX-M_at
105.7
A
0.39692
AFFX-LysX-3_at
42.8
A
0.262827
AFFX-PheX-5_at
2.8
A
0.897835
AFFX-PheX-M_at
4.4
A
0.883887
AFFX-PheX-3_at
46.6
A
0.382599
AFFX-ThrX-5_at
12.2
A
0.772364
AFFX-ThrX-M_at
47.9
A
0.425962
22282 597 Kbytes .Supplementary file Size Download File type/resource GSM76880.CEL.gz
3.4 Mb
(ftp) (http) CEL
