|
| Status |
Public on Nov 14, 2011 |
| Title |
Endometrial biopsy_ETB67_CD146-/PDGFRB+ |
| Sample type |
RNA |
| |
|
| Source name |
Endometrial biopsy_ETB67
|
| Organism |
Homo sapiens |
| Characteristics |
age (years): 26
tissue: endometrium
phase: Early Secretory
cycle day: 13
cd146 and pdgfrb expression: CD146-/PDGFRB+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
FACS analyzed sorted cell populations were subject to RNA isolation and purification, with DNAse treatment, using Pico Pure RNA Isolation Kit
|
| Label |
biotin
|
| Label protocol |
RNA was reverse transcribed/amplified into cDNA using the NuGEN FFPE kit, and sense-strand cDNA targets were created using the NuGEN WT-Ovation Exon Module, followed by fragmentation and labeling using NuGEN Encore Biotin Module for hybridization to Affymetrix Human Gene 1.0 ST arrays.
|
| |
|
| Hybridization protocol |
Microarrays were hybridized, washed, stained, and scanned according to the protocol described in the WT sense target labeling assay manual from Affymetrix (version 4; FS450_0007).
|
| Scan protocol |
Microarrays were hybridized, washed, stained, and scanned according to the protocol described in WT Sense Target Labeling Assay Manual from Affymetrix (Version 4; FS450_0007) at the UCSF Gladstone Genomics Core Facility.
|
| Data processing |
GeneSpringGX 8.0, Microarray Technology: Affymetrix.ExonExprChip.HuGene-1_0-st-v1, Summarization Algorithm: ExonRMA16, Normalization: Quantile, Baseline Transformation: median of all samples
|
| |
|
| Submission date |
Aug 02, 2011 |
| Last update date |
Nov 14, 2011 |
| Contact name |
Linda Giudice |
| E-mail(s) |
[email protected]
|
| Phone |
415-476-2039
|
| Organization name |
University of California, San Francisco
|
| Department |
OBGYN and RS
|
| Lab |
Giudice Lab
|
| Street address |
513 Parnassus Ave. HSE 1619
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94122 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE31152 |
Perivascular Human Endometrial Mesenchymal Stem Cells Express Pathways Relevant to Self-Renewal, Lineage Specification, and Functional Phenotype |
|
