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| Status |
Public on Aug 06, 2011 |
| Title |
MOLF backcross animal 4030 |
| Sample type |
SRA |
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| Source name |
PyMT-induced mammary tumor
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| Organism |
Mus musculus |
| Characteristics |
strain background: ((MOLF x PyMT) x FVB) backcross animal
tissue: MOLF x PyMT backcross mammary tumor
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| Extracted molecule |
total RNA |
| Extraction protocol |
Illumina mRNA Library Preparation Protocol (Total RNA extracted from mammary tissue, Purify mRNA by poly-A selection, Fragment mRNA, Synthesize the First Strand cDNA, Synthesize the Second Strand cDNA, Perform End Repair, Adenylate 3' ends, Ligate the Adapters, Purify the cDNA Templates on a gel to select a size range)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
mean insert size : 242.5
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| Data processing |
Illumina software pipeline ( Basecalls was done by using Illumina RTA 1.6 for GAIIx run, RTA 1.7 for HiSeq run , Alignment was done by using Illumina CASAVA1.6, CASAVA_v1.7.0 pipelines)
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| Submission date |
Aug 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Ying Hu |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
240 276 5273
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| Organization name |
NIH
|
| Department |
NCI/CCR
|
| Lab |
CBIIT
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| Street address |
9609 Medical Center Dr.
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| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platform ID |
GPL13112 |
| Series (2) |
| GSE30866 |
Gene expression of polyoma middle T antigen induced mammary tumors |
| GSE31223 |
Gene expression of polyoma middle T antigen induced mammary tumors [RNA_Seq : MOLF x PyMT] |
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| Relations |
| SRA |
SRX093385 |
| BioSample |
SAMN00714260 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM774019_NCIMouse4030_Gene_RPKM.txt.gz |
255.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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