|
| Status |
Public on May 01, 2012 |
| Title |
Cd2_R3 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 in medium and 2 μM Cd
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
growth condition: 2 μM Cd
|
| Treatment protocol |
Cells were plated on day onto 100 mm diameter Petri dishes (Corning) at a concentration of 1.5×106 cells/dish. Twenty four hours after seeding, the medium was removed and the cells were either treated with fresh medium added with CdCl2 (Cd) at a concentration of 2 µM and 10 µM, or with complete medium (control) for 24 h.
|
| Growth protocol |
HepG2 cells were routinely cultured in Opti-MEM medium (Invitrogen9, supplemented with 10% fetal bovine serum, and 1% antibiotics. Cells were kept in incubator at constant 37°C under a humidified 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 24h of treatment, the cells were washed with cold PBS and lysed in 350 µl of RLT lysis buffer (Qiagen) following manufacturer instructions
|
| Label |
Cy3
|
| Label protocol |
standard Agilent protocol
|
| |
|
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
Scanned with the Agilent G2565BA Microarray Scanner (Agilent)
|
| Description |
Gene expression after treatment with 2 μM Cd
|
| Data processing |
The fluorescence intensities on scanned images were extracted and preprocessed by Agilent Feature Extraction Software (v10.5.1.1).
|
| |
|
| Submission date |
Aug 09, 2011 |
| Last update date |
May 01, 2012 |
| Contact name |
marco fabbri |
| E-mail(s) |
[email protected]
|
| Phone |
+39 0332 78 9538
|
| Fax |
+39 0332 78 5446
|
| Organization name |
European Commission - Joint Research Centre
|
| Department |
Institute for Health and Consumer Protection
|
| Lab |
Molecular Biology and Genomics unit
|
| Street address |
Via E. Fermi, 2749
|
| City |
Ispra (VA) |
| ZIP/Postal code |
21027 |
| Country |
Italy |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE31286 |
Whole genome analysis regulation in HepG2 cells exposed to cadmium |
|
