|
| Status |
Public on Aug 19, 2012 |
| Title |
C3W53_hum |
| Sample type |
RNA |
| |
|
| Source name |
ES-E-hybrid-line3-week53
|
| Organism |
Mus musculus |
| Characteristics |
hybrid cell line: ES-E-hybrid line 3
cell type: cultured human erythroblasts x murine erythroleukemia heterospecific hybrids
cell culture age: week 53
gamma to beta globin switching phase: post-switching phase
globin expression status: stable beta globin expression.
|
| Biomaterial provider |
The University of Washington (Seattle, WA).
|
| Treatment protocol |
human chromosome 11-containing MEL cells were generated via fusing hESC-derived erythroblasts with MEL cells. Each hybrid lines were cultured for up to 68 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by extraction with a commercially available kit (Ambion) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix GeneChip® Mouse Gene 1.0 ST Arrays through a core facility in the Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
|
| Description |
Mus musculus cells with some Homo sapiens chromosomes
|
| Data processing |
Affymetrix Expression Console v1.1 RMA normalization log2
|
| |
|
| Submission date |
Aug 18, 2011 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE31523 |
Transcriptional environment and chromatin modifications interplay decides globin expression patterns. |
|
