|
| Status |
Public on Dec 14, 2023 |
| Title |
Participant2_SD_plasma_24hrs |
| Sample type |
protein |
| |
|
| Source name |
plasma
|
| Organism |
Homo sapiens |
| Characteristics |
individual: Participant2
condition: sleep deprived
time: 24hrs
tissue: plasma
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
protein |
| Extraction protocol |
Intravenous lines were placed for longitudinal collection of blood. Blood samples were centrifuged to extract plasma. A lumbar catheter was placed for longitudinal CSF sampling.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labelled SOMAmer.
|
| |
|
| Hybridization protocol |
Hybridized overnight (19 hours) at 55 C as recommended by SomaLogic.
|
| Scan protocol |
SOMAmer matching probes printed onto Agilent slides. After overnight hybridization slides were scanned on an Agilent Microarray SureScan Scanner model D.
|
| Data processing |
Standard data processing method as per SomaLogic recommended methods. Normalized and calibrated using kit control samples
quantile-normalization was applied on top of vendor-standardized and calibrated relative fluorescence unit (RFU, in log2 scale) and non-normalized and non-standardized raw data are provided in linear scale.
|
| |
|
| Submission date |
Oct 06, 2023 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Jinsheng Yu |
| E-mail(s) |
[email protected]
|
| Organization name |
Washington University School of Medicine
|
| Department |
Genetics
|
| Lab |
GTAC Lab
|
| Street address |
660 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL33741 |
| Series (1) |
| GSE244817 |
Plasma and CSF Proteomic Signatures of Acutely Sleep-Deprived Humans: An Exploratory Study |
|
