NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM783208 Query DataSets for GSM783208
Status Public on Aug 26, 2011
Title 543T_Macro
Sample type RNA
 
Source name Macro Samples
Organism Homo sapiens
Characteristics disease state: Tumor
cell type: Adeno carcinoma
Extracted molecule total RNA
Extraction protocol 50mg to 80mg of snap frozen lung tissue was added to a tube containing 1 ml of ice cold trizol and homogenized until tissue was totally homogenized. After incubation for 5 min at room temperature, 0.2ml of chloroform was added; tube was shaked vigorously by hand for 15 sec and then incubated for 3 min at room temperature. Then samples were centrifuged for 20 min at 12000g at 4C. Aqueous phase was transfered to a clean 1.5-ml tube and 1 x volume of 70% ethanol was added into the aqueous phase. Sample was applied to the Rneasy column and centrifuged at 8000g for 30 sec. Further procedure was done using Rneasy mini kit according to the manufactures suggestions including an optional 30 min DNase I treatment.
Label biotin
Label protocol 100ng total RNA was amplified using Ambion WT Expression Kit following the manufacturer’s instructions. 5.5 µg of amplification products were labeled with Affymetrix WT Terminal Labeling Kit hybridized to Affymetrix HG Gene 1.0ST chips using Affymetrix Hybridization Control Kit and washed and stained using Affymetrix Wash and Stain Kit. All steps were done following the manufacturer’s instructions.
 
Hybridization protocol Affymetrix protocol
Scan protocol Affymetrix protocol
Data processing Affymetrix Expression Console software and RMA (Robust Multichip Analysis) normalization was performed to generate the CHP files.
 
Submission date Aug 19, 2011
Last update date Apr 26, 2012
Contact name Miao Shi
E-mail(s) [email protected]
Phone 1-718-678-1035
Organization name Albert einstein College of Medicine
Street address 1301 Morris Park ave
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Platform ID GPL6244
Series (1)
GSE31552 Expression Data from human Lung tissue of Patients with Non Small Cell Lung Cancer (NSCLC)

Data table header descriptions
ID_REF
VALUE log2-rma

Data table
ID_REF VALUE
7892501 4.47447
7892502 5.81769
7892503 5.57584
7892504 9.64133
7892505 4.71099
7892506 6.46374
7892507 3.89384
7892508 6.54326
7892509 11.522
7892510 5.34214
7892511 4.52112
7892512 8.76991
7892513 4.18245
7892514 10.6333
7892515 9.92095
7892516 6.35768
7892517 7.05837
7892518 4.05482
7892519 6.11617
7892520 9.8037

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM783208_543T-8344.081811_Macro.rma-gene-default.chp.gz 256.6 Kb (ftp)(http) CHP
GSM783208_543T-8344.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap