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Status
Public on Nov 14, 2005
Title
rop10-1 mutant, no ABA control, replicate 2
Sample type
RNA
Source name
rop10-1, no ABA control
Organism
Arabidopsis thaliana Characteristics
rop10-1 in Ws background, young seedlings
Extracted molecule
total RNA
Extraction protocol
Approximately 100 mg of 7-day old seedlings were frozen and ground in liquid nitrogen. Total RNA was extracted using an TRIzol kit (Invitrogen) and purified by RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
rop10-1 seedlings mock-treated by DMSO for 4 hours in liquid medium (MS) after 7 days of growth in liquid medium (MS + 3% sucrose) at 150rpm shaking.
Data processing
Affymetrix GCOS 1.1.1 software
Submission date
Oct 14, 2005
Last update date
Oct 14, 2005
Contact name
Zhi-Liang Zheng
Organization name
Lehman College of City University of New York
Department
Biological Sciences
Street address
250 Bedford Park Blvd W
City
Bronx
State/province
NY
ZIP/Postal code
10468
Country
USA
Platform ID
GPL198 Series (1)
GSE3454 Transcriptome analysis of ROP10 GTPase-mediated abscisic acid (ABA) signaling in Arabidopsis
Data table header descriptions ID_REF VALUE GCOS 1.1.1 software-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
179.5
P
0.003595
AFFX-BioB-M_at
155.9
P
0.000052
AFFX-BioB-3_at
123
P
0.001593
AFFX-BioC-5_at
411.8
P
0.000127
AFFX-BioC-3_at
252.6
P
0.000095
AFFX-BioDn-5_at
499.8
P
0.000044
AFFX-BioDn-3_at
1927.8
P
0.000052
AFFX-CreX-5_at
4787.4
P
0.000044
AFFX-CreX-3_at
5853.3
P
0.000044
AFFX-DapX-5_at
107
P
0.000297
AFFX-DapX-M_at
290.9
P
0.001102
AFFX-DapX-3_at
326.7
P
0.000081
AFFX-LysX-5_at
22
A
0.131361
AFFX-LysX-M_at
72.1
A
0.287743
AFFX-LysX-3_at
79.1
P
0.000297
AFFX-PheX-5_at
33.8
A
0.262827
AFFX-PheX-M_at
45.6
A
0.131361
AFFX-PheX-3_at
77.5
A
0.108979
AFFX-ThrX-5_at
37.2
A
0.116113
AFFX-ThrX-M_at
79.2
P
0.002556
22810 589 Kbytes .Supplementary data files not provided
