Wild type ecotype Wassilewskija (Ws), young seedlings
Extracted molecule
total RNA
Extraction protocol
Approximately 100 mg of 7-day old seedlings were frozen and ground in liquid nitrogen. Total RNA was extracted using an TRIzol kit (Invitrogen) and purified by RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type (Ws), seedlings treated by 1µM ABA (dissolved in DMSO) for 4 hours in liquid medium (MS) after 7 days of growth liquid medium (MS + 3% sucrose) at 150rpm shaking.
