|
| Status |
Public on Jul 17, 2024 |
| Title |
AML patient group with low β-catenin levels , AML7 |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ cells from AML patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow/blood
cell type: CD34+ cells
disease state: AML
β-catenin level: low
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Trizol (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocols were used.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 μg of cRNAs were hybridized for 16 hours at 45°C, 60 rpm on Human GeneChip HG-U133 Plus 2.0 arrays (Affymetrix). The chips were washed and stained in the GeneChip Fluidics Station 450 (Affymetrix) using the EukGE-WS2v5_450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from CD34+ cells from AML patient
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 13, 2023 |
| Last update date |
Jul 17, 2024 |
| Contact name |
nevin belder |
| E-mail(s) |
[email protected]
|
| Organization name |
ankara university
|
| Street address |
gumusdere
|
| City |
ankara |
| ZIP/Postal code |
06135 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE245305 |
Genome-wide identification of beta-catenin related genes in CD34 + Hematopoietic Stem and Progenitor Cells of patients with Acute Myeloid Leukemia |
|
