|
| Status |
Public on Jan 01, 2006 |
| Title |
volunteer 90 Pre-Endotoxin Instillation Circulating Neutrophils |
| Sample type |
RNA |
| |
|
| Source name |
Neutrophil
|
| Organism |
Homo sapiens |
| Characteristics |
volunteer 90 Pre-Endotoxin Instillation Circulating Neutrophils
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 20 million airspace or peripheral blood neutrophils, previously stabilized in RNAlater (Ambion), were lysed in Trizol (Life Technologies) and total RNA isolated with Rneasy MinElute columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 3 ?g of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
volunteer 90 Pre-Endotoxin Instillation Circulating Neutrophils
|
| Data processing |
GCRMA
|
| |
|
| Submission date |
Oct 18, 2005 |
| Last update date |
Oct 18, 2005 |
| Contact name |
Christopher D Coldren |
| E-mail(s) |
[email protected]
|
| Phone |
303 724 6056
|
| Organization name |
University of Colorado School of Medicine
|
| Department |
Medicine
|
| Lab |
Pulmonary Sciences and Critical Care Medicine
|
| Street address |
12700 East 17th Place
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE2322 |
Expression changes induced by transmigration in human neutrophils |
|
