|
| Status |
Public on Nov 27, 2024 |
| Title |
RA1938M_Day6 |
| Sample type |
SRA |
| |
|
| Source name |
Serum
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: Serum
timepoint: Day6
radiation dose_gy: 7.5
gender: M
survival: Survived
|
| Treatment protocol |
Subjects were irradiated and dose measurement were performed by EMXmicro spectrometer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood samples were extracted and centrifuged to obtain serum samples for analysis
TruSeq small RNA Sample Preparation kit was used for library construction
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
177_RA1938M_SD6_S1
|
| Data processing |
Illumina NextSeq 500 generated BCL files that were converted to FastQ files
FastQ files were demultiplexed using bcl2fastq2
Genomics Workbench was used to filter and trim the fastQ files
miRbase was used to generate miRNA expression count data
Data was normalized to CPM values using TMM normalization
Assembly: NHP gene assmebly in miRbase
Supplementary files format and content: raw count data contains the count data for miRNA
|
| |
|
| Submission date |
Nov 07, 2023 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Aarti Gautam |
| E-mail(s) |
[email protected]
|
| Phone |
301-619-7683
|
| Organization name |
WRAIR
|
| Department |
Medical Readiness Systems Biology
|
| Street address |
503 Robert Grant Avenue
|
| City |
Silver Spring |
| State/province |
MD |
| ZIP/Postal code |
20910 |
| Country |
USA |
| |
|
| Platform ID |
GPL21120 |
| Series (1) |
| GSE247225 |
Cross-species conserved miRNA as biomarker of radiation injury and to predict fatality risk |
|
| Relations |
| BioSample |
SAMN38149478 |
| SRA |
SRX22408909 |
