liver sample of Wistar rat fed for 12 weeks with coconut fat diet, after overnight fast
Extracted molecule
total RNA
Extraction protocol
Wistar rats were fasted overnight and killed by CO2. A piece of the reight venreal liver lobe was removed and clamp frozen. Total RNA was isolated from the clamp-frozen liver pieces using RNeasyR mini spin columns (Qiagen). The purified RNA was routinely checked for visible signs of degradation in a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, USA), and only high quality RNA was processed.
Label
biotin
Label protocol
Approximately 3-5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
liver sample of Wistar rat fed for 12 weeks with coconut fat diet, after overnight fast
