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| Status |
Public on Nov 26, 2024 |
| Title |
K192R_ChIP_3 |
| Sample type |
SRA |
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| Source name |
brain
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| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
cell line: UTSW63
cell type: human glioblastoma
genotype: shSUCLG1 rSUCLG1 K192R
antibody: L-Lactyl-Histone H3
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Two groups with 3 biological replicates were used for sample preparation, which is performed by SimpleChIP Enzymatic Chromatin IP Kit with magnetic beads
DNA fragments were repaired, A-tailed for DNA library construction using TruSeq ChIP Library Preparation Kit by Novogene company
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
ChIP-seq reads were mapped to reference genome of Illumina iGenomes UCSC hg38 using Bowtie (version 2.2.6), and only the uniquely mapped reads were retained
SAMtools (version 0.1.19) was then used to convert files to bam format, sort, and remove PCR duplicates
Peaks were called using MACS2 (version 2.1.1) with FDR = 0.01
Assembly: hg38
Supplementary files format and content: bigWig,broadPeak(except for Input sample)
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| Submission date |
Nov 15, 2023 |
| Last update date |
Nov 26, 2024 |
| Contact name |
润华 杜 |
| E-mail(s) |
[email protected]
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| Phone |
17853142797
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| Organization name |
上海药物所
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| Street address |
上海市浦东新区
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| City |
上海 |
| ZIP/Postal code |
200120 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (2) |
| GSE247800 |
Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and glioma progression [ChIP-seq] |
| GSE248704 |
Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and glioma progression |
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| Relations |
| BioSample |
SAMN38262546 |
| SRA |
SRX22530775 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7900971_K192R_ChIP_3.bw |
154.9 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
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