|
| Status |
Public on Nov 22, 2011 |
| Title |
Drosophila_3d_Brain_CAA/G105_4 |
| Sample type |
RNA |
| |
|
| Source name |
3d female fly brain expressing CAA/G105
|
| Organism |
Drosophila melanogaster |
| Characteristics |
age: 3d
gender: female
tissue: brain
genotype: elav-GAL4; UAS-DsRed-CAA/G105 (2x)
|
| Treatment protocol |
Female fly brain tissue was dissected in cold phosphate buffered saline (PBS) and stored in Trizol reagent at -80C at 3 d.
|
| Growth protocol |
Flies were raised at 25C with 12hr/12hr light-dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total brain RNA was extracted and purified using TRIzol reagent (Invitrogen) and the RNeasy Mini system (Qiagen), and treated with RNase-free DNase I (Qiagen).
cDNA was amplifed using Ovation Pico WTA system (NuGEN)
|
| Label |
biotin
|
| Label protocol |
Encore Biotin Module (NuGEN)
|
| |
|
| Hybridization protocol |
Encore Biotin Module (NuGEN)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Age-matched control flies expressing non-toxic trinucleotide repeats
|
| Data processing |
Normalization of probe signal intensity levels across arrays was performed using the GCRMA algorithm implemented in Partek Genomic suite 6.2 beta.
|
| |
|
| Submission date |
Sep 05, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Bonini Nancy |
| Organization name |
University of Pennsylvania
|
| Street address |
415 S University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE31875 |
Gene and pathways affected by CAG-repeat RNA-based toxicity in Drosophila |
|
| Relations |
| Reanalyzed by |
GSE119084 |
