|
| Status |
Public on Sep 07, 2011 |
| Title |
hsfb1_hsfb2b_over_wt_5d_seedlings-heat32_30min-rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
hsfb1 hsfb2b double mutant_32°C_30 min
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: hsfb1 hsfb2b double mutant
age: 5d
tissue: seedling
treatment: heat (32 Celsius degree) for 30 min.
|
| Treatment protocol |
Plates were submerged into water of 32 Celsius degree for 30 minutes.
|
| Growth protocol |
All plants were grown in Gamborg B5 (2% sucrose) agar media in 16hr light / 8hr dark cycle in 23 Celsius degree. Many plants of the mutant and wild type were grown in same one plate for each replicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy3
|
| Label protocol |
1 µg of total RNA was used for labeling procedure using QickAmp Labeling kit (Two-color) (Agilent).
|
| |
|
| Channel 2 |
| Source name |
Wild-type (Col-0) _32°C_30min
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: wild type
age: 5d
tissue: seedling
treatment: heat (32°C) for 30 min.
|
| Treatment protocol |
Plates were submerged into water of 32 Celsius degree for 30 minutes.
|
| Growth protocol |
All plants were grown in Gamborg B5 (2% sucrose) agar media in 16hr light / 8hr dark cycle in 23 Celsius degree. Many plants of the mutant and wild type were grown in same one plate for each replicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy5
|
| Label protocol |
1 µg of total RNA was used for labeling procedure using QickAmp Labeling kit (Two-color) (Agilent).
|
| |
|
| |
| Hybridization protocol |
Hybridized using Gene Expression Hybridization kit (Agilent) and washed by Gene Expression wash pack (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
|
| Description |
Biological replicate 1 of 4. harvested after heat treatment. Many mutant and wild-type plants were grown in a same plate.
|
| Data processing |
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction and LOWESS normalization. After that, signal of each probe was divided by median value of filter-passed probes.
|
| |
|
| Submission date |
Sep 06, 2011 |
| Last update date |
Sep 07, 2011 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7299 |
| Series (2) |
| GSE31883 |
Functional analysis of HSFB transcription factors [32°C] |
| GSE31888 |
Functional analysis of Arabidopsis HSFB transcription factor |
|
