|
| Status |
Public on Nov 20, 2024 |
| Title |
ICC_JFE_HS_405NT |
| Sample type |
RNA |
| |
|
| Source name |
Normal
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal
Sex: F
age: 75
tnm: pT3pNxpMx
|
| Treatment protocol |
Each frozen specimen was cut at 5μm and stained with hematein-eosin-saffron at two opposite ends and analyzed by an expert in liver pathology (CG) to evaluate semi-quantitatively the proportion of cancer cells, the degree of necrosis and fibrosis, and the percent cellularity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the mirVana miRNA isolation kit (Thermo Fisher Scientific, France).
|
| Label |
biotin
|
| Label protocol |
Biotinylated double strand cDNA targets were prepared, starting from 1 to 20 ng of total RNA with the NuGEN Ovation Pico WTA System V2 Kit (Cat # 3302) followed by the NuGEN Encore Biotin Module Kit (Cat # 4200) according to manufacturer recommendations.
|
| |
|
| Hybridization protocol |
Following fragmentation and end-labeling, 4.7 μg of cDNAs were hybridized for 16 hours at 45oC on GeneChip® Human Transcriptome Array 2.0 (Affymetrix).
|
| Scan protocol |
The chips were scanned with the GeneChip® Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm.
|
| Description |
Gene expression data from normal tissue
|
| Data processing |
CEL files were further processed with Affymetrix Expression Console software version 1.3.1 using Robust Multi-array Average (RMA) algorithms with default settings at the gene level.
|
| |
|
| Submission date |
Nov 23, 2023 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Sylvie JOB |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM
|
| Street address |
UMR-S 1193 INSERM/Université Paris Saclay Hôpital Paul Brousse
|
| City |
Villejuif |
| ZIP/Postal code |
94800 |
| Country |
France |
| |
|
| Platform ID |
GPL17585 |
| Series (1) |
| GSE248516 |
Immunosuppressive inflammation driven by LPS/TLR4 signalling in hepatocellular carcinoma with low immune cell infiltration |
|
