|
| Status |
Public on Jan 12, 2012 |
| Title |
Kidney, control diet administrated for 24d, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney, fed control AIN-93G diet for 24d
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
strain: Wistar
tissue: Kidney
age: 8 weeks
diet: control AIN-93G diet for 24d
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent (Invitrogen Life Technologies Corp.), then purified with an RNeasy mini kit (Qiagen K.K., Tokyo, Japan).
|
| Label |
biotin
|
| Label protocol |
biotinylated aRNA was obtained from 100 ng of purified total RNA using a GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
CEL files were quantified with the distribution free weighted (DFW) method, using the statistical language R and Bioconductor.
|
| |
|
| Submission date |
Sep 07, 2011 |
| Last update date |
Jul 21, 2015 |
| Contact name |
Yuji Nakai |
| Organization name |
Hirosaki University
|
| Department |
Institute of Regional Innovation
|
| Lab |
Section of Food Sciences
|
| Street address |
2-1-1, Yanagawa
|
| City |
Aomori |
| State/province |
Aomori |
| ZIP/Postal code |
038-0012 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE31973 |
Effect of a High Phosphorus Diet on Renal Gene Expressions in Rat |
|
