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| Status |
Public on Nov 26, 2024 |
| Title |
K192R_4 |
| Sample type |
SRA |
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| Source name |
brain
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| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
cell line: UTSW63
cell type: human glioblastoma
genotype: shSUCLG1 rSUCLG1 K192R
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| Extracted molecule |
total RNA |
| Extraction protocol |
the total RNA of these two groups with 3 biological replicates was extracted by RNeasy Plus Kits and used for construction of the library.
The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification using QIAseq Stranded RNA Library Kit by Novogene company
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
RNA-seq reads were mapped to reference genome of Illumina iGenomes UCSC hg38 using TopHat version 2.1.0.
Mapped reads were summarized for each gene using featureCounts version 1.5.0.
Differential expression analysis was implemented using edgeR version 3.16.5.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using edgeR version 3.16.5.
Assembly: hg38
Supplementary files format and content: Tab-delimited text files include RPKM values for each Sample
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| Submission date |
Nov 27, 2023 |
| Last update date |
Nov 26, 2024 |
| Contact name |
润华 杜 |
| E-mail(s) |
[email protected]
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| Phone |
17853142797
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| Organization name |
上海药物所
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| Street address |
上海市浦东新区
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| City |
上海 |
| ZIP/Postal code |
200120 |
| Country |
China |
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| Platform ID |
GPL16791 |
| Series (2) |
| GSE248703 |
Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and glioma progression [RNA-Seq] |
| GSE248704 |
Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and glioma progression |
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| Relations |
| BioSample |
SAMN38457679 |
| SRA |
SRX22659109 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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