|
| Status |
Public on Dec 06, 2023 |
| Title |
T_at_E14.5_M_embryo |
| Sample type |
RNA |
| |
|
| Source name |
Tongue from E14.5 mouse embryos
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
developmental stage: E14.5
tissue: Tongue
region: Medial
|
| Treatment protocol |
Pregnant mice were euthanized by cervical dislocation after inhalation anesthesia. Embryos were collected into fresh Hanks’ balanced salt solution (HBSS) at 4℃ and maxillofacial tissues were dissected from the head under a stereomicroscope.
|
| Growth protocol |
Timed-pregnant ICR mice were kept under a 12 h light/12 h dark cycle with free access to standard laboratory feed and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated from cerebellums of each individual animal using TRIzol Reagent (Thermo Fisher Scientific) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Low Input Quick Amp Labeling Kit (Agilent) was employed according to the manufactuer's instruction.
|
| |
|
| Hybridization protocol |
The cDNA was hybridized to a 60K Agilent 60-mer oligomicrroarray accoding to the manufacturer's instruction.
|
| Scan protocol |
A hybridized microarray slides were scanned by an Agilent scanner.
|
| Description |
Gene expression data from 4 embryos
|
| Data processing |
Relative hybridization intensities and background hybridization values were calculated using Agilent Feature Extraction Software (9.5.1.1).
|
| |
|
| Submission date |
Dec 06, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Taisuke Hani |
| E-mail(s) |
[email protected]
|
| Organization name |
The Nippon Dental University, School of Life Dentistry at Tokyo
|
| Department |
Pathology
|
| Street address |
1-9-20
|
| City |
Fujimi, Chiyoda-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
155-0032 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE249490 |
The expression data from mandibular arch and tongue primordia in mouse embryos. |
|
