|
| Status |
Public on Oct 01, 2011 |
| Title |
RST_sample5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
recurrent spindle tumour
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
initial tumour onset (days): 47 +/- 8.6
tumor regression (days): 35 +/- 11
tumor recurrence (days): 116 +/- 110
age at tissue collection (days): 265 +/1 107
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy RNA Isolation Kit from Qiagen using the manufacturer's protocol
|
| Label |
Cy3
|
| Label protocol |
500ug of total RNA was labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit using the manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
universal mouse reference RNA
|
| Organism |
Mus musculus |
| Characteristics |
sample type: universal mouse reference RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy RNA Isolation Kit from Qiagen using the manufacturer's protocol
|
| Label |
Cy5
|
| Label protocol |
500ug of total RNA was labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit using the manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
hybridization was performed using the Agilent In situ Hybridization Kit using the manufacturer's protocol
|
| Scan protocol |
scanned on an Agilent G2565BA DNA Scanner
|
| Description |
Sample name: RJ423
Raw data file: july3_251486819145_S01_GE2-v5_95_Feb07_1_3.txt
|
| Data processing |
Agilent Feature Extraction Software v9.5
|
| |
|
| Submission date |
Sep 15, 2011 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Roger A Moorehead |
| E-mail(s) |
[email protected]
|
| Phone |
519-824-4120 (54950)
|
| Fax |
519-767-1450
|
| Organization name |
University of Guelph
|
| Department |
Department of Biomedical Sciences
|
| Street address |
50 Stone Rd East
|
| City |
Guelph |
| State/province |
Ontario |
| ZIP/Postal code |
N1G2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE32152 |
Mammary Tissue: Wild type mammary glands vs IGF-IR induced mammary tumors vs IGF-IR independent tumors |
|
