|
| Status |
Public on Sep 25, 2024 |
| Title |
LB PF rep1 |
| Sample type |
SRA |
| |
|
| Source name |
primary fibroblast cells
|
| Organism |
Ailuropoda melanoleuca |
| Characteristics |
treatment: LB PF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA per sample was extracted using Rneasy mini plus kit (Qiagen). A total amount of 2 μg RNA per sample was used for the library construction.
Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina following the manufacturer’s recommendations.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
PF1
|
| Data processing |
Gene-level expression was estimated as transcripts per million (TPM) using the high-speed transcript quantification tool Kallisto.
Assembly: CPB_AME_v1
Supplementary files format and content: tab-delimited text files include TPM and counts for each sample.
|
| |
|
| Submission date |
Dec 15, 2023 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Yan Li |
| E-mail(s) |
[email protected]
|
| Phone |
17628288812
|
| Organization name |
Chengdu Research Base of Giant Panda Breeding
|
| Street address |
375#Panda Road,Northern Suburb
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
613000 |
| Country |
China |
| |
|
| Platform ID |
GPL34015 |
| Series (1) |
| GSE250292 |
Establishment of Giant Panda Induced Pluripotent Stem Cells |
|
| Relations |
| BioSample |
SAMN38859732 |
| SRA |
SRX22906943 |
