|
| Status |
Public on Feb 14, 2024 |
| Title |
KO_R.DraR1,no_stress,rep1 |
| Sample type |
SRA |
| |
|
| Source name |
cells
|
| Organism |
Deinococcus radiodurans |
| Characteristics |
tissue: cells
genotype: KO_R.DraR1-1
|
| Growth protocol |
tryptone glucose yeast extract (TGY) liquid media at 30◦C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Trizol method.1% denaturing agarose gels were used to assess RNA degradation and contamination. RNA concentration and RNA integrity were measured using an RNA Nano 6000 Assay kit and Bioanalyzer 2100 system.
A total of 3G RNA text per sample was used as the input material for library preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The library preparations were sequenced on an Illumina Hiseq platform and paired-end reads were generated.
Assembly: Deinococcus radiodurans R1
Supplementary files format and content: table delimited text files include FPKM values for each sample
|
| |
|
| Submission date |
Dec 20, 2023 |
| Last update date |
Feb 14, 2024 |
| Contact name |
Chenxiang Shi |
| E-mail(s) |
[email protected]
|
| Organization name |
Zhejiang University
|
| Street address |
Sandun Zhen Yuhangtang Road 866
|
| City |
Hangzhou |
| State/province |
Zhejiang province |
| ZIP/Postal code |
1 |
| Country |
China |
| |
|
| Platform ID |
GPL31018 |
| Series (1) |
| GSE251690 |
Characterization of a novel N4 -methylcytosine restriction-modification system in Deinococcus radiodurans |
|
| Relations |
| SRA |
SRX22919957 |
| BioSample |
SAMN38879806 |
