|
| Status |
Public on Feb 01, 2024 |
| Title |
Control (biological replicate 1) |
| Sample type |
SRA |
| |
|
| Source name |
bone-marrow
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone-marrow
cell type: HSC
treatment: None
|
| Treatment protocol |
Treated mice received anti-NEO1/anti-CD62p/anti-CD47/anti-cKIT, HSCs were isolated at day 9.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 1,000 total HSCs (KLS FLT3–CD34–CD150+) were FACS-sorted from aged control mice or aged mice that received antibody-conditioning 9 days earlier and immediately added to lysis buffer
Libraries were prepared using Takara SMART-Seq v4 Ultra low Input RNA kit and sequencing was performed with NovaSeq with approximately 20 million paired reads per sample by MedGenome Inc.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
HSCs isolated from Control animals
|
| Data processing |
For 'Gene_Biotype', only 'protein_coding' genes are included
Assembly: The paired-end reads were aligned to the reference Mouse genome downloaded from Ensembl database (genome-build GRCm38.p6; genome-version GRCm38; genome-build-accession NCBI: GCA_000001635.8)
Supplementary files format and content: tab-delimited text file includes raw counts for each sample
|
| |
|
| Submission date |
Dec 26, 2023 |
| Last update date |
Feb 01, 2024 |
| Contact name |
Jason Ross |
| Organization name |
Stanford University
|
| Street address |
265 Campus Drive
|
| City |
Palo Alto |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
|
| Relations |
| SRA |
SRX22920579 |
| BioSample |
SAMN38882176 |
