|
| Status |
Public on Dec 01, 2012 |
| Title |
Colon_HAM_2 |
| Sample type |
RNA |
| |
|
| Source name |
Colon_HAM
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: colon
diet: HAM
|
| Treatment protocol |
rats fed different diets as described in Conlon et al
|
| Growth protocol |
Distal colon tissues sampled from 11 week old rats
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extrac ed from distal colon tissue
|
| Label |
biotin
|
| Label protocol |
taking 100ng of total RNA from the aforementioned extractions, poly-A RNA controls 214 were added, double-stranded cDNA was synthesised with random hexamers tagged 215 with a T7 promoter sequence using Affymetrix cDNA synthesis and amplification kit 216 and the product cleaned using the Affymetrix Sample Cleanup module
|
| |
|
| Hybridization protocol |
using the 225 GeneChip Hybridization, Wash and Stain Kit, the cRNA target was hybridized onto 226 the arrays in an Affymetrix Hybridization Oven.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
HAM_14D.CEL
|
| Data processing |
Data were processed using bioconductor and R. Data were normalized using RMA.
|
| |
|
| Submission date |
Sep 22, 2011 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Robert Dunne |
| Organization name |
CSIRO
|
| Street address |
Locked Bag 17
|
| City |
North Ryde |
| State/province |
New South Wales |
| ZIP/Postal code |
1760 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE32312 |
Gene Expression in Rats Fed a Western Diet |
|
