|
| Status |
Public on Oct 20, 2011 |
| Title |
PBMC TRIF-/- LPS |
| Sample type |
RNA |
| |
|
| Source name |
PBMC from TRIF deficient patient
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: LPS
concentration: 10ng/ml
time point: 2hrs
cell type: PBMC
disease state: herpes simplex virus type 1 (HSV1) encephalitis (HSE) TRIF deficient patient
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in RLT + 1% β-Mercaptoethanol, RNA were isolated using Qiagen Rneasy mini kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
X5617835153_B.AVG_signal
|
| Data processing |
The data were normalised using quantile normalisation with in R. PALO (p<0.05 present in at least one) were used to filter the data.
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Elisabeth Israelsson |
| E-mail(s) |
[email protected]
|
| Organization name |
AstraZeneca, IMED RIA
|
| Department |
Translational Biology
|
| Street address |
Pepparedsleden 1
|
| City |
Molndal |
| ZIP/Postal code |
SE-431 83 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE32390 |
In vitro responses of PBMC and fibroblasts from patients with TRIF deficiency after TRIF dependent and independent stimuli |
|
