Strain: C57BL6 Mouse Brain - pons total RNA (MoPo.Feml) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoPo.Feml.sig21 Cell type Mouse Brain - pons Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoPo.Feml_sig21.5611F.a-20 10/24/2005 669173 15872 QC Passed MoPo.Feml_sig21.5570F.f-20 9/28/2005 417791 11525 QC Passed MoPo.Feml_sig21.5570W.c-20 9/13/2005 311048 12054 QC Passed MoPo.Feml_sig21.5570W.b-20 8/26/2005 622967 11838 QC Passed Run group: Total Beads successfully sequenced - 2020979 Processed Signatures - 18810
