|
| Status |
Public on Jun 28, 2012 |
| Title |
Glom_LD_470 |
| Sample type |
RNA |
| |
|
| Source name |
Glomeruli from control living donor
|
| Organism |
Homo sapiens |
| Characteristics |
patient number: 3
disease status: control
tissue: Glomeruli from kidney biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy mini kit from Qiagen, following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from the glomerular compartment of human pre-transplant living donor control kidney
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 10 were used for the normalization.
|
| |
|
| Submission date |
Oct 04, 2011 |
| Last update date |
Jun 28, 2012 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL14663 |
| Series (2) |
| GSE32591 |
Expression data from human with lupus nephritis (LN) |
| GSE32592 |
Human and mouse lupus nephritis cross-species transcriptional analysis |
|
