|
| Status |
Public on Oct 11, 2012 |
| Title |
whole lung Brown Norway rat, age PN7, replicate d, in utero Saline-exposed |
| Sample type |
RNA |
| |
|
| Source name |
rat whole lung
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Brown Norway
age (postnatal days): 7
exposure in utero: Saline
tissue: whole lung
beadchip sentrix barcode: 5067474016
beadchip section: H
short label: B.7.d.+
selected for reproducibility analysis: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from whole lung tissue and primary lung cells was isolated using Trizol reagent (Invitrogen, Burlington, ON, Canada) according to the manufacturer’s instructions. RNA was resuspended in 1x RNASecure (Ambion, Austin, TX, USA) and treated with the Turbo DNase-free kit (Ambion, Austin, TX, USA) to remove any traces of DNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
FKN085
|
| Data processing |
The data were standard background corrected and quantile normalized using the R Bioconductor package lumi.
|
| |
|
| Submission date |
Oct 05, 2011 |
| Last update date |
Oct 11, 2012 |
| Contact name |
Alvin T. Kho |
| E-mail(s) |
[email protected]
|
| Phone |
617-919-2182
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Street address |
320 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE32622 |
In utero allergen exposure reprograms primary developmental patterns of lung gene expression and influences respiratory phenotype in rat models of asthma susceptibility. |
|
