strain: Lewis age (postnatal days): 14 exposure in utero: Saline tissue: whole lung beadchip sentrix barcode: 5067474025 beadchip section: D short label: L.14.d.+ selected for reproducibility analysis: Yes
Extracted molecule
total RNA
Extraction protocol
RNA from whole lung tissue and primary lung cells was isolated using Trizol reagent (Invitrogen, Burlington, ON, Canada) according to the manufacturer’s instructions. RNA was resuspended in 1x RNASecure (Ambion, Austin, TX, USA) and treated with the Turbo DNase-free kit (Ambion, Austin, TX, USA) to remove any traces of DNA.
Label
biotin
Label protocol
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Standard Illumina scanning protocol
Description
FKN101
Data processing
The data were standard background corrected and quantile normalized using the R Bioconductor package lumi.
In utero allergen exposure reprograms primary developmental patterns of lung gene expression and influences respiratory phenotype in rat models of asthma susceptibility.
Data table header descriptions
ID_REF
VALUE
Standard background corrected and quantile normalized using the R Bioconductor package lumi in logarithm base 2 scale.
