|
| Status |
Public on Apr 01, 2013 |
| Title |
uninvolved.tissue-544 |
| Sample type |
RNA |
| |
|
| Source name |
uninvolved.tissue
|
| Organism |
Homo sapiens |
| Characteristics |
patient_number: 544
Sex: F
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were extracted from human patients during surgical resection of lung tumors.
RNA was extracted with Trizol followed by DNAse I treatment and purification (RNeasy RNA purification kit, Qiagen). The purified RNA was quantified with Nanodrop and checked by Bioanalyzer for the quality.
|
| Label |
biotin
|
| Label protocol |
Biotin-NTP mix with T7 enzyme mix was used to make biotinylated cRNAs. The labeled cRNAs were purified, quantified, and checked again with Bioanalyzer.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4155430039_B
|
| Data processing |
Data normalized by quantile normalization with limma in R. Probes with present/absent call ≥ 0.05 assessed by Illumina Beadstudio software were treated as absent in analysis.
|
| |
|
| Submission date |
Oct 06, 2011 |
| Last update date |
Apr 01, 2013 |
| Contact name |
David Quigley |
| Organization name |
UCSF
|
| Department |
Helen Diller Comprehensive Cancer Center
|
| Lab |
Ashworth Lab
|
| Street address |
1450 Third St. Room 207
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE32665 |
Rewiring of human lung cell lineage and mitotic networks in lung adenocarcinomas |
|
