|
| Status |
Public on Apr 25, 2024 |
| Title |
hiPSC-CM, scrambled siRNA, differentiation 3 |
| Sample type |
SRA |
| |
|
| Source name |
cardiomyocyte
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: cardiomyocyte
genotype: WT
treatment: scrambled siRNA
|
| Treatment protocol |
Cells were transfected with Lipofectamine 2000 (Life Technologies)-encapsulated siRNAs at a concentration of 100 nM.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was issolated using the miRNease Mini Kit (Qiagen). 500 ng of total RNA were used for sequencing librabry construction.
Library was prepared utilizing the 'Novogene NGS RNA Library Prep Set (PT042)* (Novogene). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq X Plus |
| |
|
| Data processing |
Alignment using HISAT2
Quantification using featureCounts
Assembly: Human reference (GRCh38) datasets
Supplementary files format and content: csv file containing raw counts
|
| |
|
| Submission date |
Mar 07, 2024 |
| Last update date |
Apr 25, 2024 |
| Contact name |
Maximilian Fuchs |
| E-mail(s) |
[email protected]
|
| Organization name |
Fraunhofer Institute for Toxicology and Experimental Medicine ITEM
|
| Department |
Cardiovascular Research
|
| Street address |
Nikolai-Fuchs-Str. 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL34284 |
| Series (1) |
| GSE261129 |
Circular RNA circZFPM2 regulates cardiomyocyte hypertrophy and survival |
|
| Relations |
| BioSample |
SAMN40301528 |
| SRA |
SRX23875027 |
