|
| Status |
Public on Sep 28, 2012 |
| Title |
LCS-CCA-23 |
| Sample type |
RNA |
| |
|
| Source name |
LCS-CCA-23
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver Tumor Tissue
disease state: Combined Hepatocellular Cholangiocarcinoma (CHC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from fresh frozen tissues using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. RNA integrity for each sample was confirmed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
cDNA was labelled with TdT using the WT Terminal Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Biotinylated cDNA was hybridized to GeneChip Gene 1_0 st arrays. Arrays were washed and stained on an Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanning was carried out with the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from CHC
|
| Data processing |
Image analysis was perfomed using the GeneChip Operating Software (GCOS, Affymetrix). Background correction, normalization and probe summarization was done with the robust multichip average (RMA) algorithm implemented in the Expression Console software (Affymetrix).
|
| |
|
| Submission date |
Oct 11, 2011 |
| Last update date |
Sep 28, 2012 |
| Contact name |
Xin Wei Wang |
| E-mail(s) |
[email protected]
|
| Phone |
240-760-6858
|
| Organization name |
National Cancer Institute
|
| Department |
Laboratory of Human Carcinogenesis
|
| Lab |
Liver Carcinogenesis Unit
|
| Street address |
37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4255 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE32879 |
Integrative Transcriptomic Profiling reveals Hepatic Stem-like Phenotype and Interplay of EMT and miR-200c in Intrahepatic Cholangiocarcinoma [mRNA] |
| GSE32958 |
Integrative Transcriptomic Profiling reveals Hepatic Stem-like Phenotype and Interplay of EMT and miR-200c in Intrahepatic Cholangiocarcinoma |
|
