|
| Status |
Public on Oct 10, 2012 |
| Title |
Wild type heart at 3.5 weeks old 1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type mouse heart 3.5weeks old
|
| Organism |
Mus musculus |
| Characteristics |
background strain: 129/Sv
gender: male
genotype/variation: Hspa4+/+
|
| Growth protocol |
Total RNA was extracted from heart ventricles of 3.5-week-old Hspa4+/+ and Hspa4-/- males (n = 3 mice for each genotype).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Using the WT Target Labeling Kit (Affymetrix), cDNA was synthesized according to the manufacture´s recommendations. Global gene expression analysis was applied using the Gene-Chip® Mouse Gene 1.0 ST arrays (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed for 18 hours at 60 rpm and 45°C in the GeneChip® Hybridization Oven 640 followed by washing and staining in Gene Chip® Fluidics Station 450 (Affymetrix),
|
| Scan protocol |
the arrays were scanned using the GeneChip® scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data of 3.5-week-old wild type heart
|
| Data processing |
Data were analyzed using R (Version 2.13.0) and Bioconductor using the rma-function of package Affy (affy_1.30.0) and the CDF-package mogene10stv1mmentrezgcdf_14.1.0 from Brainarray (http://brainarray.mbni.med.umich.edu)
|
| |
|
| Submission date |
Oct 11, 2011 |
| Last update date |
Oct 10, 2012 |
| Contact name |
belal Awd Mohamed |
| E-mail(s) |
[email protected]
|
| Organization name |
Medical university, Goettingen
|
| Department |
Institute of Human Genetics
|
| Lab |
108
|
| Street address |
Heinrich Dueker Weg 12
|
| City |
Goettingen |
| State/province |
Lower Saxony |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13730 |
| Series (1) |
| GSE32885 |
Loss of heat shock protein HSPA4 aggravates pressure overload-induced myocardial damage |
|
