Normalization Method: Spatial Normalization. Ref: Wilson DL, Buckley MJ, Helliwell CA, Wilson IW (2003) New normalization methods for cDNA microarray data. Bioinformatics 19, 1325-32.
Normalized log2 ratio of medians defined as CH1 divided by CH2
BACK_TRANSFORM_VALUE
Ratio of medians defined as CH1 divided by CH2 back transformed from normalised log
CH1_FG
Channel 1 median feature pixel intensity (635nm)
CH1_BG
Channel 1 median feature background intensity
CH2_FG
Channel 2 median feature pixel intensity (532nm)
CH2_BG
Channel 2 median feature background intensity
RATIO
Unnormalized, untransformed ratio of medians (Ch1/Ch2 in unswapped sample, Ch2/Ch1 in swapped sample)
FLAG
the type of flag associated with a feature. 0 denotes satisfactory features, <0 denotes un-satisfactory features that were excluded from normalisation and analysis