|
| Status |
Public on Nov 27, 2024 |
| Title |
NAc_Coca2 |
| Sample type |
SRA |
| |
|
| Source name |
C57Bl6J male mice, Nucleus Accumbens
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6J
tissue: Nucleus Accumbens
Sex: male
treatment: Cocaine CPP
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were isolated with QIAGEN miRNseasy Kits.
Total RNA library
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Nac
Exploration in conditioned place preference chamber with cocaine (7.5 mg/kg, i.p.) administraion
NAc_Counts.txt
|
| Data processing |
Bulk RNA-seq reads were aligned with STAR 2.7.1a to the reference sequence (mm10 from UCSC). Mouse reference was used for gene annotation and counting (gencode M27). Uniquely mapped reads were quantified using featureCounts using protein coding genes by gene.
Assembly: Mouse mm10
Supplementary files format and content: tab-delimited text files raw counts for each sample
|
| |
|
| Submission date |
Mar 14, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Stefano Berto |
| E-mail(s) |
[email protected]
|
| Phone |
4696626889
|
| Organization name |
Medical University of South Carolina
|
| Department |
Neuroscience
|
| Lab |
BE208
|
| Street address |
68 President Street
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29403 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE261663 |
Total RNA-seq from mice of NAc and mPFC |
|
| Relations |
| BioSample |
SAMN40464144 |
| SRA |
SRX23955595 |
