|
| Status |
Public on Sep 28, 2012 |
| Title |
LCS-CCA-MI18 |
| Sample type |
RNA |
| |
|
| Source name |
LCS-CCA-18
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver Tumor Tissue
disease state: Combined Hepatocellular Cholangiocarcinoma (CHC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from fresh frozen tissues using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. RNA integrity for each sample was confirmed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 ul of the fivefold diluted sample preparation reaction. All hybridization reactions were incubated at 65ﾂｰC for a minimum of 18 h.
|
| Scan protocol |
Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer's instructions. For each assay, a high-density scan (600 fields of view) was performed.
|
| Description |
Gene expression data from CHC
|
| Data processing |
The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using code count normalization according to manufacturer's protocol (Nanostring).
|
| |
|
| Submission date |
Oct 13, 2011 |
| Last update date |
Sep 28, 2012 |
| Contact name |
Xin Wei Wang |
| E-mail(s) |
[email protected]
|
| Phone |
240-760-6858
|
| Organization name |
National Cancer Institute
|
| Department |
Laboratory of Human Carcinogenesis
|
| Lab |
Liver Carcinogenesis Unit
|
| Street address |
37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4255 |
| Country |
USA |
| |
|
| Platform ID |
GPL14732 |
| Series (2) |
| GSE32957 |
Integrative Transcriptomic Profiling reveals Hepatic Stem-like Phenotype and Interplay of EMT and miR-200c in Intrahepatic Cholangiocarcinoma [miRNA] |
| GSE32958 |
Integrative Transcriptomic Profiling reveals Hepatic Stem-like Phenotype and Interplay of EMT and miR-200c in Intrahepatic Cholangiocarcinoma |
|
